Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology)

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Plant Physiol. Almagro L. Cyclodextrins increase phytosterol and tocopherol levels in suspension cultured cells obtained from mung beans and safflower. Yue W. Medicinal plant cell suspension cultures: Pharmaceutical applications and high-yielding strategies for the desired secondary metabolites. Saffron: An old medicinal plant and a potential novel functional food.

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Methyl jasmonate and miconazole differently affect arteminisin production and gene expression in Artemisia annua suspension cultures. Plant Biol. Ahlawat S. Modulation of artemisinin biosynthesis by elicitors, inhibitor, and precursor in hairy root cultures of Artemisia annua L. Plant Interact. Zamboni A. Elicitor-induced resveratrol production in cell cultures of different grape genotypes Vitis spp. Durante M. Chilton M. Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant-root cells.

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Balestra G. Increased susceptibility to Pseudomonas syringae pv. Syringae and Pseudomonas viridiflava of kiwi plants having transgenic rolABC genes and its inheritance in the T1 offspring. Atti Giornate Scientifiche S. Istituto Sperimentale Frutticoltura; Rome, Italy: Risultati preliminari sulla caratterizzazione morfo-fisiologica di cultivar di actinidia Actinidia deliciosa A. Druart P. Genetic transformation of cherry trees.

Vahdati K. Gentile A. Morphological and physiological effects of rolABC genes into Citrus genome. Kiwi Inf. Welander M. Genetic transformation of the apple rootstock M26 with the RolB gene and its influence on rooting. Growth characteristics of apple cultivar Gravenstein plants grafted onto the transformed rootstock M26 with rolA and rolB genes under non-limiting nutrient conditions.

Firson A. Agrobacterium transformation of Actinidia kolomikta. Mezzetti B. Genetic transformation in strawberry and raspberry for improving plant productivity and fruit quality. Bell R. Kaneyoshi J. Characteristics of transgenic trifoliate orange Poncirus trifoliata Raf. Eapen S. Advances in development of transgenic pulse crops. Fontana G. Genetic transformation in the grain legume Cicer arietinum L. Genetic transformation of grain legumes: Phaseolus vulgaris L.

Soybean Glycine max L. Atif R. Gene transfer in legumes. In: Luttge U. Progress in Botany. Iantcheva A. Transformation of leguminous plants to study symbiotic interactions. Estrada-Navarrete G. Fast, efficient and reproducible genetic transformation of Phaseolus spp. Nova-Franco B. In vitro meristematic organogenesis and plant regeneration in bean Phaseolus vulgaris L. Citadin C. Genetic engineering in Cowpea Vigna unguiculata : History, status and prospects.

GM Crops. Kumar M. An update on genetic modification of Chickpea for increased yield and stress tolerance. Hnatuszko-Konka K. Phaseolus vulgaris -recalcitrant potential. Nguyen A. An approach to overcoming regeneration recalcitrance in genetic transformation of lupins and other legumes. Plant Cell Tissues Organ Cult. Das A. Advances in biotechnological applications in three important food legumes. Indurker S. Genetic transformation of chickpea Cicer arietinum L. Sahoo D. Enhanced salinity tolerance in transgenic mungbean overexpressing Arabidopsis antiporter NHX1 gene. Singh P.

Mishra S. Development of transgenic imazapyr-tolerant cowpea Vigna unguiculata Plant Cell Rep. Pigeaire A. Transformation of a grain legume Lupinus angustifolius L. Atkins C. Consequences of transforming narrow leafed lupin Lupinus angustifolius [L. Barker S. Regeneration selection improves transformation efficiency in narrow-leaf lupin. PCTOC ; — Wijayanto T. Significant reduction of fungal disease symptoms in transgenic lupin Lupinus angustifolius expressing the anti-apoptotic baculovirus gene p Tabe L. Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume.

Transgenic dry bean tolerant to the herbicide glufosinate ammonium. Crop Sci. Rech E. High-efficiency transformation by biolistics of soybean, common bean and cotton transgenic plants. Collado R. Epicotyl sections as targets for plant regeneration and transient transformation of common bean using Agrobacterium tumefaciens. In Vitro Cell.

Tiwari S. Expression of a synthetic cry1EC gene for resistance against Spodoptera litura in transgenic peanut Arachis hypogaea L. Expression of delta-endotoxin Cry1EC from an inducible promoter confers insect protection in peanut Arachis hypogaea L. Chu Y. Bcl-xL transformed peanut Arachis hypogaea L. Krishna G. Progress in genetic engineering of peanut Arachis hypogaea L. Das S. Agrobacterium -mediated genetic transformation in lentil Lens culinaris Medik. Plant Tissue Cult. Advances in faba bean genetics and genomics. Jaganathan D.

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Plant Cell Physiol. Are homeobox knotted-like genes and cytokinins the leaf architects? Overexpression of KNAT1 in lettuce shifts leaf determinate growth to a shoot-like indeterminate growth associated with an accumulation of isopentenyl-type cytokinins. Kakimoto T. Takei K. Identification of genes encoding adenylate isopentenyltransferase, a cytokinin biosynthesis enzyme, in Arabidopsis thaliana. Yanai O. Jasinski S.

KNOX action in Arabidopsis is mediated by coordinate regulation of cytokinin and gibberellin activities. Sakamoto T. Shani E. Cytokinin regulates compound leaf development in tomato. KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem. Genes Dev. Tanaka-Ueguchi M.

Over-expression of a tobacco homeobox gene, NTH15 , decreases the expression of a gibberellin biosynthetic gene encoding GA oxidase. Bolduc N. Di Giacomo E. Scofield S. Clouse S. Sun Y. Integration of brassinosteroid signal transduction with the transcription network for plant growth regulation in Arabidopsis. Tsuda K. Genome-wide study of KNOX regulatory network reveals brassinosteroid catabolic genes important for shoot meristem function in rice. Gendron J. Brassinosteroids regulate organ boundary formation in the shoot apical meristem of Arabidopsis. Cano-Delgado A.

Mele G. Testone G. The peach Prunus persica L. Batsch genome harbours 10 KNOX genes, which are differentially expressed in stem development, and the class 1 KNOPE1 regulates elongation and lignification during primary growth. Hake S. The role of knox genes in plant development. Cell Dev. Kerstetter R. Sequence analysis and expression patterns divide the maize knotted1 -like homeobox genes into two classes.

Furumizu C. PLoS Genet. Poethig R. Vegetative phase change and shoot maturation in plants. Hanke M. In: Books G. Genes, Genomes and Genomics. Volume 1. Global Science Books Ltd. Layne D. The Peach: Botany, Production and Uses. Bitonti M. Distinct nuclear organization, DNA methylation pattern and cytokinin distribution mark juvenile, juvenile-like and adult vegetative apical meristems in peach Prunus persica L.

Batsch J. Giannino D. Isolation and characterization of a maintenance DNA-methyltransferase gene from peach Prunus persica [L. Isolation and molecular characterisation of the gene encoding the cytoplasmic ribosomal protein S28 in Prunus persica [L. Van Nocker S. Breeding better cultivars, faster: Applications of new technologies for the rapid deployment of superior horticultural tree crops. Gordon D. Preformation in vegetative buds of Prunus persica : Factors influencing number of leaf primordia in overwintering buds. Tree Physiol. Reinoso H. Dormancy in peach Prunus persica flower buds.

Anatomy of bud development in relation to phenological stage. Yamane H. Batsch]: A summary of recent studies. Hyun Y. Competence to flower: Age-controlled sensitivity to environmental cues. Zhang L. Involvement of miR in the regulation of vegetative phase change in plants. Wang J. Jia X. Sgamma T. In vitro plant culture system induces phase transition in fruit-bearing plants. Albani M. Comparative analysis of flowering in annual and perennial plants. Wells C. Hong Y. Floral induction and flower formation—The role and potential applications of miRNAs. Identification and characterization of Prunus persica miRNAs in response to UVB radiation in greenhouse through high-throughput sequencing.

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Sabbadini S. Increased frequency of polyethylene glycol induced protoplast fusion by dimethyl-sulphoxide. Helgeson, J. Somatic hybridization of wild Solanum species with potato: a potential source of diversity for breeders. Pudoc, Wageningen, pp. Hibi, T. High-speed electro-fusion and electro-transfection of plant protoplasts by a continuous flow electro-manipulator. Hinnisdaels, S. Horn, M. Plant regeneration from protoplasts of embryogenic suspension cultures of orchardgrass Dactylis glomerata L. Transgenic plants of orchardgrass Dactylis glomerata L.

Plant protoplasts : a biotechnological tool for plant improvement (Book, ) []

Hunt, G. A medium and simplified procedure for growing single cells from Solanum species. Ichikawa, H. Transfer of mitochondria through protoplast fusion. Imamura, J. X-ray irradiation promoted asymmetric somatic hybridization and molecular analysis of the products. Imbrie-Milligan, C. Microcallus growth from maize protoplasts. Planta 58— Ishii, S. Factors influencing protoplast viability of suspension-cultured rice cells during isolation process. Enzymes for the isolation of protoplasts. Itoh, K. In situ hybridization with species-specific DNA probes gives evidence for asymmetric nature of Brassica hybrids obtained by X-ray fusion.

Jacobsen, J. Control of transient expression of chimaeric genes by gibberellic acid and abscisic acid in protoplasts prepared from mature barley aleurone layers. Jahne, A. Regeneration of fertile plants from protoplasts derived from embryogenic cell suspension of barley Hordeum vulgare L. Jaiswal, S. Plant regeneration from cotyledon protoplasts of Brassica carinata.

Jenes, B. Plant regeneration from protoplast derived calli in rice Oryza sativa L. Joersbo, M. Electroporation: Mechanism and transient expression, stable transformation and biological effects in plant protoplasts. Jones, C. Science Jones, H. Transient gene expression in electroporated Solanum protoplast. Jones, M. Van der Plas and J. Van Aartrijk eds. Jouanneau, J. In plant protoplasts, the spontaneous expression of defense reactions and the responsiveness to exogenous elicitors are under auxin control.

Jourdan, P. Improved protoplast culture and stability of cytoplasmic traits in plants regenerated from leaf protoplasts of cauliflower Brassica oleracea ssp. Junker, B. Kameya, T. Induction of hybrids through somatic cell fusion with dextran sulphate and gelatin. Kao, H. Efficient plant regeneration from hypocotyl protoplasts of broccoli Brassica oleracea L. Ogura cytoplasmic male sterility and triazine tolerant Brassica napus cv. Westar produced by protoplast fusion. Kao, K. Plant protoplast fusion and growth of intergeneric hybrids cells. A method for fusion of plant protoplasts with polyethylene glycol.

In: Plant Tissue Culture Methods. Gamborg and L. Wetter eds. Chromosomal behavior in somatic hybrids of soybean-Nicotiana glauca. Fusion of plant protoplasts at the interface of a glucose and a sucrose-polyethylene glycol solution. A method for high-frequency intergeneric fusion of plant protoplasts. Nutritional requirements for growth of Vicia hajstana cells and protoplasts at a very low population density in liquid media. Plant regeneration from mesophyll protoplasts of alfalfa. Pflanzenphysiol — Fusion of plant protoplasts—techniques. Karesch, H. Arabidopsis thaliana : protocol for plant regeneration from protoplasts.

Direct gene transfer to protoplasts of Arabidopsis thaliana. Kartzke, S. Molecular analysis of transgenic plants derived from transformations of protoplasts at various stages of the cell cycle. Kemble, R. Use of protoplast fusion systems to study organelle genetics in a commercially important crop. Cell Biol. Kirti, P. Rapid plant regeneration through organogenesis and somatic embryogenesis from cultured protoplasts of Brassica juncea. Kitamura, Y. Isolation and culture of protoplasts from cell suspension cultures of Duboisia myoporoides with subsequent plant regeneration. Klercker, J. Methode zur Isolterung lebender Protoplasten.

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Recovery of somatic embryos and plantlets from protoplasts cultures of Larix x eurolepis. Regeneration of isolated barley microspores in conditioned media and trials to characterize the responsible factor. Enhancement of transformation rates in higher plants by low-dose irradiation: Are DNA repair systems involved in the incorporation of exogenous DNA into the plant genome.

Kohn, H. Somatic hybrids in tobacco mediated by electrofusion. Koop, H. Regeneration of plants from individually cultivated protoplasts using an improved microculture system. Koornneef, M. Breeding of a tomato genotype readily accessible to genetic manipulation. Krens, F. In vitro transformation of plant protoplasts with Ti-plasmid DNA. Transfer of cytoplasm from new Beta CMS sources to sugar beet by asymmetric fusion. Krogaard, H. Effect of cellulase fractionation on the viability of cultured barley Hordeum vulgare protoplasts.

Krumbiegel, G. Selection of somatic hybrids after fusion of protoplasts from Datura innoxia Mill. Kumar, A. Protoplast fusion: A novel approach to organelle genetics in higher plants. Kunitake, H. Somatic embryogenesis and plant regeneration from protoplasts of asparagus Asparagus officinales L.

Plant regeneration from cell culture-derived protoplasts of statice Limonium perezii Hubbard. Kyozuka, J. High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Plant regeneration from protoplasts of indica rice: genotypic differences in culture response. Production of cytoplasmic male sterile rice Oryza sativa by cell fusion. Lang, H. Callus formation from mesophyll protoplasts of Fagus sylvatica L.

Larkin, P. Nurse culture of low numbers of Medicago and Nicotiana protoplasts using calcium alginate beads. Lee, B. Transient gene expression in aleurone protoplasts isolated from developing caryopses of barley and wheat. Lee, C. Interspecific gametosomatic hybridization in Petunia hybrida Plant Cell Rep. Lee, L. Plant regeneration from indica rice Oryza sativa L. Lee, N. Protoplast isolation and callus production from leaves of tissue cultured Vitis spp. Lee-Stadelmann, O. Plasmolysis of Glycine max mesophyll cells: the use of octylguanidine and its implications in protoplast isolation.

Lehminger-Mertens, R. Plant regeneration from pea protoplasts via somatic embryogenesis. Lenee, P. Isolation and culture of sunflower protoplasts Helianthus annuus L. Li, Z. Efficient plant regeneration from rice protoplasts in general medium. High frequency generation of fertile transgenic rice plants after PEG-mediated protoplast transformation. Improved protoplast culture and agarose media.

Loudon, P. Studies of protoplast culture and plant regeneration from commercial and rapid-cycling Brassica species. Liihrs, R. Initiation of morphogenic cell-suspension and protoplast cultures of barley Hordeum vulgare L. Planta 71— Lurquin, P. Eschericia coli plasmid pBR insertion into protoplasts and into their nuclei. Lynch, P. Electrofusion of protoplasts from celery Apium graveolens L. Lyznik, L. Stable transformation of maize: the impact of feeder cells on protoplast growth and transformation efficiency. Maas, C. The combination of a novel stimulatory element in the first exon of the maize Shrunken-1 gene with the following intron 1 enhances reporter gene expression up to fold.


Marchesi, M. Effect of repeated DNA sequences on direct gene transfer in protoplasts of Nicotiana plumbaginifolia. Mascarenhas, D.

Protoplast isolation

Intron-mediated enhancement of heterologous gene expression in maize. Masson, J. Plant regeneration from protoplasts of diploid potato derived from crosses of Solanum tuberosum with wild Solanum species. Masuda, K. Callus formation and plant regeneration from rice protoplasts purified by density gradient centrifugation. Matthews, D. A protoplast to plant system in roses. Medgyesy, P. Selection and analysis of cytoplasmic hybrids. Interspecific chloroplast recombination in a Nicotiana somatic hybrids. Mehrle, W. Effects of microgravitation on electrofusion of plant cell protoplasts.

Determination of physical membrane properties of plant cell protoplasts via the electrofusion technique: prediction of optimal fusion yields and protoplast viability. Meijer, E. Transgenic rice cell lines and plants: Expression of transferred chimeric genes. Melchers, G. Somatic hybridization of plants by fusion of protoplasts I. Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res.

Plant Protoplasts for Cell Fusion and Direct DNA Uptake: Culture and Regeneration Systems

Menczel, L. Isolation of somatic hybrids by cloning Nicotiana heterokaryons in nurse cultures. Planta 29— Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum x Nicotiana knightiana : correlation of resistance to Nicotiana tabacum plastids. Meyer, Y. Auxin-induced regulation of protein synthesis in tobacco mesophyll protoplast cultivated in vitro , 1: Characteristics of auxin-sensitive proteins. Auxin-induced regulation of protein synthesis in tobacco mesophyll protoplast cultivated in vitro , 2: Time course and level of auxin control.

Meyer, P. A genomic DNA segment from Petunia hybrida leads to increased transformation frequencies and simple integration patterns. Michel, W. Mieth, H. Phytoalexin production by isolated soybean protoplasts. Mitchell, J. Plant regeneration from haploid suspension and protoplast cultures from isolated microspores of maize. Mock, H. Cell wall synthesis in carrot cells: compari- son of suspension-cultured cells and regenerating protoplasts. An improved system for the obtention of fertile regenerants via maize protoplasts isolated from a highly embryogenic suspension culture.

Moyne, A. Callus and embryoid formation from protoplasts of Helianthus annuus. Response to chilling of tomato mesophyll protoplasts. Mussel, H. Ethylene synthesis during protoplast formation from leaves of Avena sativa. Myers, J. Genotype-dependent whole plant regeneration from protoplasts of red clover Trifolium pratense L. Nagata, T. A novel cell-fusion method of protoplasts by polyvinyl alcohol.

Naturwissenschaften — Surface charge of protoplasts and their significance in cell-cell interaction. Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta 12— Naton, B. Improved electrofusion of protoplasts of varied fusibility by selective pairing: application of asymmetric breakdown of plasma membranes. Negrutiu, I. Hybrid genes in the analysis of transformation conditions. Setting up a simple method for direct gene transfer to protoplasts. Hybrid genes in the analysis of transformation conditions: II. Transient expression vs stable transformation - analysis of parameters influencing gene expression levels and transformation efficiency.

Niedz, R. Multifactor analysis of environmental preconditioning of tomato seedlings on protoplasts culture and development. Niizeki, M. Somatic hybridization in rice x soybean. Nyman, M. Plant regeneration from strawberry Fragaria x Ananassa mesophyll protoplasts. Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and L. Oard, J. Chimeric gene expression using maize intron in cultured cells of breadwheat.

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Plant regeneration from root callus protoplasts of sour cherry Prunus cerasus L. Shoot regeneration from leaf mesophyll protoplasts of wild pear Pyrus Communis var. Electroporationmediated improvement of plant regeneration of colt cherry Prunus avium x pseudocerasus protoplasts. Bartlett pear Pyrus communis L. Long-term effect of electroporation on enhancement of growth and plant regeneration of colt cherry Prunus avium x pseudocerasus protoplasts.

Ohyama, K. Uptake of exogenous DNA by plant protoplasts. Okamura, M. Inhibiting effect of ammonium ion in protoplast culture of some Asteraceae plants. Plant and Cell Physiol. Oliviera, M. Plant regeneration from protoplasts of long-term callus. Hayword kiwifruit. Omirulleh, S. Activity of a chimeric promoter with doubled CaMV 35S enhancer element in protoplast-derived cells and transgenic plants in maize. Paszkowski, J. Direct gene transfer to plants. EMBO J. Gene targeting in plants. Plant gene vectors and genetic transformation: DNA-mediated direct gene transfer to plants.

Vasil and J. Schell eds. Patat-Ochatt, E. Plant regeneration from protoplasts of apple rootstocks and scion varieties Malus x domestica Borkh. Patnaik, G. Importance of enzyme purification for increased plating efficiency and plant regeneration from single protoplasts of Petunia parodii. Pental, D. Somatic hybridization of Nicotiana tabacum and Petunia hybrida.

Recovery of plants with P. A selection method for the synthesis of triploid hybrids by fusion of microspore protoplasts n with somatic cell protoplasts 2n. Perl, A. Ethylene and in vitro culture of potato: suppression of ethylene generation vastly improves protoplast yield, plating efficiency and transient expression of an alien gene. Protoplast-fusion-derived Solanum cybrids: Application and phylogenetic limitations.

Phillips, R. A simple technique for single-cell cloning of crown gall tumor tissue: Petunia protoplast regeneration without exogenous hormones. Piastuch, W. Chromosomal analysis of Nicotiana asymmetric somatic hybrids by dot blotting and in situ hybridization.

Piccirilli, M. Lotus tenuis Wald. Pilet, P. Comparison between maize root cells and their respective regenerating protoplasts: Wall polysaccharides. Pillai, V. Plant regeneration from mesophyll protoplasts of Centaurea cyanus , Senecio x hybridus and Callistephus chinensis. Poste, G. Membrane fusion. Acta — Potrykus, I. Gene transfer to plants: Assessment of published approaches and results. Poulsen, G.

Regeneration of plants from hypocotyl protoplasts of rapeseed Brassica napus L. Preiszner, J. Characterization of morphological variation and cold resistance in interspecific somatic hybrids between potato Solanum tuberosum L. Euphytica 37— Prioli, L. Plant regeneration and recovery of fertile plants from protoplasts of maize Zea mays L. Prives, J. Increased membrane fluidity precedes fusion of muscle cells. Pua, E. Somatic embryogenesis and plant regeneration from hypocotyl protoplasts of Brassica juncea L. Punja, Z.

Isolation, culture and plantlet regeneration from cotyledon and mesophyll protoplasts of two pickling cucumber Cucumis sativus L. Pupilli, F. Plant regeneration from callus and protoplast cultures of Lotus pedunculatus Cay. Rasheed, J. Root hair protoplasts of Lotus corniculatus L. Renaudin, J. Sequential hormone requirement for growth and organogenesis of Petunia hybrida protoplasts-derived calli. Rethmeier, N. Improvement of regeneration of Lycopersicon pennellii protoplasts by decreasing ethylene production.

Rhodes, C. Plant regeneration from protoplasts isolated from embryogenic maize cell cultures. Biotechnology 6: 56— Genetically transformed maize plants from protoplasts. Science — Riggs, C. Stable transformation of tobacco by electroporation: evidence for plasmid concatenation. Rodenburg, K. Single-stranded DNA used as an efficient new vehicle for transformation of plant protoplasts.

Russell, J. Recovery of plants from leaf protoplasts of hybrid-poplar and aspen clones. Ryan, C. Oligosaccharide signals in plants: A current assessment. Sakai, T. Intergeneric transfer of cytoplasmic male sterility between Raphanus sativus cros line and Brassica napus through cytoplast-protoplast fusion. Sala, C. Saleem, M. Stabilizing corn leaf protoplasts with n-Propyl Gallate.

Salmenkallio, M. Regulation of a-amylase promoter by gibberellic acid and absisic acid in barley protoplasts transformed by electroporation. Sauvadet, M. A protoplast-to-plant system in chrysanthenum: differential responses among several commercial clones. Schaffler, E. Single cell nurse culture of tobacco protoplasts: physiological analysis of conditioning factors.

Schieder, O. Protoplast fusion and generation of somatic hybrids. Schmitz, P. Regeneration and evacuolation of protoplasts from mesophyll, hypocotyl and petioles from Helianthus annuus L. Schnabl, H. Immobilization of plant protoplasts. Schocher, R. Co-transformation of unlinked foreign genes into plants by direct gene transfer. Biotechnology 4: Viability factors in plant suspension cultures—some properties. Schwall, M. Zein promoter activity in transiently transformed protoplasts from maize. Schweiger, H. Individual selection, culture and manipulation of higher plant cells.

Senda, M. Induction of cell fusion of plant protoplasts by electrical stimulation. Effect of temperature on membrane fluidity on protoplast fusion. Shea, E. Structural analysis of the cell walls regenerated by carrot protoplasts. Shepard, J. Mesophyll cell protoplasts of potato.

Genetic transfer in plants through interspecific protoplast fusion. Shillito, R. Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species. High frequency direct gene transfer to plants. Biotechnology 3: — Regeneration of fertile plants from protoplasts of elite inbred maize. Shimamoto, K. Fertile transgenic rice plants regenerated from transformed protoplasts. Shneyour, Y. A simple Feeder-Layer technique for the plating of plant cells and protoplasts at low density.

Somatic embryogenesis and regeneration of plantlets in protoplast cultures from somatic embryos of coffee Coffea canephora P. Sihachakr, D. Plant regeneration from protoplast culture of sweet potato Ipomoea batatas Lam. Sikdar, S. Sim, G. Direct somatic embryogenesis from protoplasts of Citrus mitis Blanco. Transfer of resistance against Phoma lingam to Brassica napus by asymmetric somatic hybridization combined with toxin selection. Genet — Smidsrod, O.

Alginate as immobilization matrix for cells. Trends in Biotechnology 8: 71— Smith, M. Plasmolytic behavior of the donor cell may affect protoplast response. Analysis of chloroplast and mitochondrial DNAs in asymmetric somatic hybrids between tobacco and carrot. Somers, D. Immunological evidence for transfer of the barley nitrate reductase structural gene to Nicotiana tabacum by protoplasts fusion.

Song, J. Direct embryogenesis from single mesophyll protoplasts in alfalfa Medicago sativa L. Spangenberg, G. Somatic hybridization by microfusion of defined protoplast pairs in Nicotiana : morphological; genetic, and molecular characterization. Organelle transfer by microfusion of defined protoplast-cytoplast pairs. Steinbrenner, B. Viability factors in plant suspension cultures—a novel bioassay. Stenger, D. Kinetics of ultrastructural changes during electrically-induced fusion of human erythrocytes.

Sun, C. Regeneration of haploid and dihaploid plants from protoplasts of supersweet sh2sh2 corn. Szabados, L. Fusion between interphase and mitotic plant protoplasts. Cell Res. Tada, Y. Efficient gene introduction into rice by electroporation and analysis of transgenic plants: Use of electroporation buffer lacking chloride ions. Tagu, D. Inheritance of two foreign genes cointroduced Petunia hybrida by direct gene transfer. Takabe, I. Isolation of tobacco mesophyll cells in intact and active state. Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Takamizo, T.

Plant regeneration from suspension culture derived protoplasts of tall fescue Festuca arundinacea Schreb. Tan, M. Regeneration of leaf mesophyll protoplasts of tomato cultivars L. Tanno-Suenaga, L. Tautorus, T. Somatic embryogenesis from immature and mature zygotic embryos and embryo regeneration from protoplasts in black spruce Picea martian Mill. Tempelaar, M. Fusion characteristics of plant protoplasts in electric fields. K Modulation and direction of the electrofusion response in plant protoplasts. Terada, R. Plantlet regeneration from somatic hybrids of rice Oryza sativa L.

Teulieres, C. Isolation of protoplasts from different Eucalyptus species and preliminary studies on regeneration. Theodoropoulos, P. Progress in leaf protoplast isolation and culture from virus-free axenic shoot cultures of Vitis vinifera L. Thompson, J. Enhanced protoplast division in rice Oryza sativa L. Thompson, G. Thomzik, J. Transfer and segregation of triazine tolerant chloroplasts in Brassica napus L.

Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology) Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology)
Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology) Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology)
Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology) Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology)
Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology) Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology)
Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology) Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology)
Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology) Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology)
Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology) Plant Protoplasts: A Biotechnological Tool for Plant Improvement (Outline Studies in Biology)

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